Multifunctional Effects of Green Synthesized Silver Nanoparticles on Glioblastoma Multiform Cancer Cells

Hoseinizadeh, Nima; Kiarad, Farzaneh; Kiani, Zahra; Sadeghi, Abolfazl; Taherpour, Azadeh; Shakibaie, Mehdi

doi:10.22068/ijmse.4109

Volume 22, Issue 4 (December 2025) IJMSE 2025, 22(4): 56-64 | Back to browse issues page

‎ 10.22068/ijmse.4109

Mendeley

Zotero

RefWorks

Hoseinizadeh N, Kiarad F, Kiani Z, Sadeghi A, Taherpour A, Shakibaie M. Multifunctional Effects of Green Synthesized Silver Nanoparticles on Glioblastoma Multiform Cancer Cells. IJMSE 2025; 22 (4) :56-64
URL: http://ijmse.iust.ac.ir/article-1-4109-en.html

Multifunctional Effects of Green Synthesized Silver Nanoparticles on Glioblastoma Multiform Cancer Cells

Nima Hoseinizadeh

Abstract: (2711 Views)

Glioblastoma multiforme is an aggressive brain tumor with limited therapeutic options. This study evaluated the multifunctional anticancer effects of curcumin-synthesized silver nanoparticles (curcumin-AgNPs) on the U-87 glioblastoma cell line. Curcumin-AgNPs were biosynthesized using curcumin as a reducing and stabilizing agent and characterized by ultraviolet–visible spectroscopy (UV–Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). Cytotoxicity was assessed by MTT assay. The mRNA expression of apoptosis- and epithelial–mesenchymal transition (EMT)-related genes was quantified by real-time PCR. DLS and TEM analyses revealed curcumin-AgNPs with sizes of 56.27±4.59 nm and 22±3 nm, respectively. Curcumin-AgNPs reduced U-87 MG cell viability in a dose- and time-dependent manner. Analysis of apoptosis-related genes showed an increased BAX/BCL2L1 ratio. Additionally, FN1 and VIM were downregulated to 0.48- and 0.60-fold, respectively, indicating inhibitory effects on EMT and the metastatic potential of U-87 MG cells. These findings indicated that curcumin-AgNPs exhibit cytotoxic, pro-apoptotic, and EMT-modulating effects in U-87 MG cells, highlighting their potential as a multifunctional nanoplatform for glioblastoma research. Further studies are required to elucidate their underlying mechanisms.

Keywords: anti-cancer, glioblastoma, silver nanoparticles, curcumin, EMT